Optical Saturation as a Versatile Tool to Enhance Resolution in Confocal Microscopy

Confocal optical microscopy

Confocal optical microscopy is a technique for increasing the contrast of microscope images, particularly in thick specimens. By restricting the observed volume, the technique keeps overlying or nearby scatterers from contributing to the detected signal. The price for this is that the instrument must observe only one point at a time (in the scanning laser version) or a group of separated points...

HIGH - RESOLUTION , MULTIPLE OPTICAL MODE CONFOCAL MICROSCOPE : II . ThEORETICAL ASPECTS OF CONFOCAL TRANSMISSION MICROSCOPY

Much analysis of microscope image formation is based upon the so-called weak scaucring approximation in which an image is linearly related to an object by a convenient convolution property which greatly simplifies reconstruction. This approximation is often invalid in tmnsmission microscopy. A better model of transmission must account for multiple scauered components in the light distribution. ...

Reflecting optical system to increase signal intensity in confocal microscopy

In fluorescence mode confocal microscopes, only 0.02% of emitted signal can be detected in best case. So, we proposed reflecting optical system to increase signal intensity detected in photon detector. In this paper, we evaluate the proposed reflecting optical system using optical transfer function. To evaluate the proposed system, we used the modeling method based on wave optics. We first calc...

Reflectance confocal microscopy of optical phantoms

A reflectance confocal scanning laser microscope (rCSLM) operating at 488-nm wavelength imaged three types of optical phantoms: (1) 100-nm-dia. polystyrene microspheres in gel at 2% volume fraction, (2) solid polyurethane phantoms (INO Biomimic(TM)), and (3) common reflectance standards (Spectralon(TM)). The noninvasive method measured the exponential decay of reflected signal as the focus (z(f...

Optical-Resolution Photoacoustic Microscopy